This registry of copies of Thomas Jefferson’s Manual of Parliamentary Practice of 1801 is a bibliographic inventory that identifies known and surviving copies of the Manual printed by Samuel Harrison Smith. The Smith printing warrants priority attention as this first edition formed the basis of Jefferson’s ongoing activities regarding parliamentary law into the years of his presidency (Alexander 2021) and the second and final edition authorized by Jefferson, published in 1812. Based on analysis of Jefferson’s correspondence with Smith, it is estimated that 100 copies of the first edition of the Manual were printed in February 1801. Of these 100 estimated copies of the Manual printed in 1801, a total of seventy (70) can be verified as currently remaining in existence, with repository location or private ownership known. The registry includes a memo analyzing and describing the project and its finding, “Jefferson's Manual of 1801: A Bibliographic Inventory of Copies Currently in Existence and Known to the Historical Record.” The tables of the Registry provide information on existing copies of the 1801 Manual with known whereabouts (Table A), known copies with unknown whereabouts (Table B), and other possible remaining copies which are recorded with previous ownership but which cannot be presently traced (Table C).